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Space-timE RePresentation, Imaging

and cellular dynamics of

molecular COmplexes


The main objective of the SERPICO team (INRIA Rennes - Bretagne Atlantique) in collaboration with STED: Space-Time imaging of cellular dynamics of organelles and EnDomenbranes at UMR 144 CNRS Institut Curie ("Subcellular Structure and Cellular Dynamics" Unit / PICT (Cell and Tissue Imaging Facilities) - IBiSA) and Nikon imaging Centre@Institut Curie-CNRS is to decipher the dynamic coordination and organization of molecular complexes at the single cell level. We focus on the cellular and molecular mechanisms of membrane traffic involved in the biogenesis of specialized organelles in epidermal cells with main functions in the immune system and in skin pigmentation and photoprotection. The mathematical theories and algorithms are mainly developed to identify other molecular processes in fundamental biology but they have also a strong potential for applications in biotechnology and medicine: disease diagnosis, detection of genomic instabilities, deterioration of cell cycle, epigenetic mechanisms and cancer prevention. Multidimensional and multimodal light microscopy combined with GFP (Green Fluorescence Protein) tagging has taken a prominent role in life science research due to its ability to study biomolecules within the entire cell and particular cell compartments or domains. We plan to investigate new imaging techniques and processing methods, mathematical models, and algorithms to build an integrated imaging approach that bridges the resolution gaps between the molecule and the whole cell, in space and time. We address the following themes:

  • image superresolution/image denoising to preserve cell integrity (photo-toxicity versus exposure time) and image analysis in multidimensional microscopy
  • spatio-temporal organization modelling of molecular species and multiscale architectures in light and electron microscopy
  • computational simulation, modelling, dynamic comparison and classification of molecules trafficking at different spatial and temporal scales / biophysical model assimilation for dynamic representation in videomicroscopy and prediction in biology

Experiments in vivo for la large class of stimuli (e.g. micro-patterning, SiRNA, drugs) will be conducted to confirm the biological hypotheses and to discern wheter the changes in expression have a role in the mechanisms under study.

The SERPICO team and the UMR144 CNRS Institut Curie Unit / PICT (Cell and Tissue Imaging Facilities) - IBiSA) are partners of France-BioImaging, the distributed coordinated French infrastructure and electronic cellular BioImaging dedicated to innovation, training and transfer ("Investissement d'Avenir" / Institut Curie).